Protocols related to P450 and reductase(s), including their expression and assays, were displayed. I encourage
P450 researchers to share important articles or protocols related to these
proteins.
LITERATURE
Guengerich FP, Martin MV, Sohl CD, Cheng Q. Measurement of cytochrome P450 and NADPH-cytochrome P450 reductase. Nat Protoc. 2009;4(9):1245-51. doi: 10.1038/nprot.2009.121. Epub 2009 Aug 6 (http://www.ncbi.nlm.nih.gov/pubmed/19661994).
Phillips, Ian R., Shephard, Elizabeth A., Ortiz de Montellano, Paul R. (Eds.) Cytochrome P450 Protocols. 3rd ed. Published by Humana Press, Totowa, NJ, 2013. 319 pages, ISBN ISBN 978-1-62703-320-6 (http://www.springer.com/new+%26+forthcoming+titles+(default)/book/978-1-62703-320-6).
WEBSITES
Cytochrome P450 Homepage: http://drnelson.uthsc.edu/CytochromeP450.html
CYPED: http://www.cyped.uni-stuttgart.de/cgi-bin/CYPED5/index.pl
FCPD: http://p450.riceblast.snu.ac.kr/index.php?a=view
KITS & INSTRUMENTS
CYP450 Assay Systems - Promega
Cytochrome P450 Activity Kits & Assays - Life technologies
Cytochrome c Reductase (NADPH) Assay Kit - Sigma (Catalog Number: CY0100)
Instrument popular for P450 spectrum:
SpectraMax M2 & M2e Multi-Mode Microplate Reader. Molecular Devices. (http://www.moleculardevices.com/Products/Instruments/Microplate-Readers/Multi-Mode-Readers/SpectraMax-M2-M2e.html).
LITERATURE
Guengerich FP, Martin MV, Sohl CD, Cheng Q. Measurement of cytochrome P450 and NADPH-cytochrome P450 reductase. Nat Protoc. 2009;4(9):1245-51. doi: 10.1038/nprot.2009.121. Epub 2009 Aug 6 (http://www.ncbi.nlm.nih.gov/pubmed/19661994).
Phillips, Ian R., Shephard, Elizabeth A., Ortiz de Montellano, Paul R. (Eds.) Cytochrome P450 Protocols. 3rd ed. Published by Humana Press, Totowa, NJ, 2013. 319 pages, ISBN ISBN 978-1-62703-320-6 (http://www.springer.com/new+%26+forthcoming+titles+(default)/book/978-1-62703-320-6).
WEBSITES
Cytochrome P450 Homepage: http://drnelson.uthsc.edu/CytochromeP450.html
CYPED: http://www.cyped.uni-stuttgart.de/cgi-bin/CYPED5/index.pl
FCPD: http://p450.riceblast.snu.ac.kr/index.php?a=view
KITS & INSTRUMENTS
CYP450 Assay Systems - Promega
Cytochrome P450 Activity Kits & Assays - Life technologies
Cytochrome c Reductase (NADPH) Assay Kit - Sigma (Catalog Number: CY0100)
Instrument popular for P450 spectrum:
SpectraMax M2 & M2e Multi-Mode Microplate Reader. Molecular Devices. (http://www.moleculardevices.com/Products/Instruments/Microplate-Readers/Multi-Mode-Readers/SpectraMax-M2-M2e.html).